Virus:Article Title: Design of an intracellular aptamer-based fluorescent biosensor to track burden in Escherichia coli.
Article Snippet: .. Key Resources Table REAGENT or RESOURCE SOURCE Trends in Biotechnology, M IDENTIFIER Bacterial and virus strains DH10β NEB # C3019 BL21(DE3) NEB # C2527 Biological samples PURExpress® In Vitro Protein Synthesis Kit NEB # E6800 E. coli S30 Extract System for Circular DNA Promega # L1020 Murine RNase inhibitor NEB # M0314 Chemicals, peptides, and recombinant proteins Chloramphenicol Merck # C1919 Ampicillin Merck # A0166 DFHBI-1T SLS # SML2697-5MG HBC530 Tocris Bioscience # 7277/60U IPTG Merck # I6758 Arabinose Merck # A91906-25G-A HSL Merck # K3007 L-Rhamnose Fluorochem # F492388-25G X-Gal Thermo Fisher # R0404 Oligonucleotides Ultramers IDT N/A Recombinant DNA T7 fluorogenic RNA aptamer library This study N/A J23118 fluorogenic RNA aptamer library This study N/A pBADwt fluorogenic RNA aptamer library This study N/A pBADmut fluorogenic RNA aptamer library This study N/A Htpg1 fluorogenic RNA aptamer library This study N/A M2-VioB-mCherry Ceroni et al 2015 [3] N/A M3-VioB-mCherry Ceroni et al 2015 [3] N/A M4-VioB-mCherry Ceroni et al 2015 [3] N/A pRHA-LacZ Ceroni et al 2018 [19] N/A Software and algorithms FlowJo v10 BD https://www.flowjo.com/ Other Tecan - Infinite M Nano + Monochromator SLS # MIC9266 ClarioSTAR plate reader BMG Labtech Attune CytPix Flow Cytometer Invitrogen Eclipse Ti2-E inverted microscope Nikon onth 2025, Vol. xx, No. xx 17 Experimental model and study participants details Bacterial strains E. coli strains DH10β (K-12 F- λ- araD139 Δ(araA-leu)7697 Δ(lac)X74 galE15 galK16 galU hsdR2 relA rpsL150(StrR) spoT1 deoR φ80dlacZΔM15 endA1 nupG recA1 e14- mcrA Δ(mrr hsdRMS mcrBC)) and BL21(DE3) (F−ompT gal dcm lon hsdSB(rB−mB−) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS)) were used. ..
In Vitro:Article Title: Design of an intracellular aptamer-based fluorescent biosensor to track burden in Escherichia coli.
Article Snippet: .. Key Resources Table REAGENT or RESOURCE SOURCE Trends in Biotechnology, M IDENTIFIER Bacterial and virus strains DH10β NEB # C3019 BL21(DE3) NEB # C2527 Biological samples PURExpress® In Vitro Protein Synthesis Kit NEB # E6800 E. coli S30 Extract System for Circular DNA Promega # L1020 Murine RNase inhibitor NEB # M0314 Chemicals, peptides, and recombinant proteins Chloramphenicol Merck # C1919 Ampicillin Merck # A0166 DFHBI-1T SLS # SML2697-5MG HBC530 Tocris Bioscience # 7277/60U IPTG Merck # I6758 Arabinose Merck # A91906-25G-A HSL Merck # K3007 L-Rhamnose Fluorochem # F492388-25G X-Gal Thermo Fisher # R0404 Oligonucleotides Ultramers IDT N/A Recombinant DNA T7 fluorogenic RNA aptamer library This study N/A J23118 fluorogenic RNA aptamer library This study N/A pBADwt fluorogenic RNA aptamer library This study N/A pBADmut fluorogenic RNA aptamer library This study N/A Htpg1 fluorogenic RNA aptamer library This study N/A M2-VioB-mCherry Ceroni et al 2015 [3] N/A M3-VioB-mCherry Ceroni et al 2015 [3] N/A M4-VioB-mCherry Ceroni et al 2015 [3] N/A pRHA-LacZ Ceroni et al 2018 [19] N/A Software and algorithms FlowJo v10 BD https://www.flowjo.com/ Other Tecan - Infinite M Nano + Monochromator SLS # MIC9266 ClarioSTAR plate reader BMG Labtech Attune CytPix Flow Cytometer Invitrogen Eclipse Ti2-E inverted microscope Nikon onth 2025, Vol. xx, No. xx 17 Experimental model and study participants details Bacterial strains E. coli strains DH10β (K-12 F- λ- araD139 Δ(araA-leu)7697 Δ(lac)X74 galE15 galK16 galU hsdR2 relA rpsL150(StrR) spoT1 deoR φ80dlacZΔM15 endA1 nupG recA1 e14- mcrA Δ(mrr hsdRMS mcrBC)) and BL21(DE3) (F−ompT gal dcm lon hsdSB(rB−mB−) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS)) were used. ..
Recombinant:Article Title: Design of an intracellular aptamer-based fluorescent biosensor to track burden in Escherichia coli.
Article Snippet: .. Key Resources Table REAGENT or RESOURCE SOURCE Trends in Biotechnology, M IDENTIFIER Bacterial and virus strains DH10β NEB # C3019 BL21(DE3) NEB # C2527 Biological samples PURExpress® In Vitro Protein Synthesis Kit NEB # E6800 E. coli S30 Extract System for Circular DNA Promega # L1020 Murine RNase inhibitor NEB # M0314 Chemicals, peptides, and recombinant proteins Chloramphenicol Merck # C1919 Ampicillin Merck # A0166 DFHBI-1T SLS # SML2697-5MG HBC530 Tocris Bioscience # 7277/60U IPTG Merck # I6758 Arabinose Merck # A91906-25G-A HSL Merck # K3007 L-Rhamnose Fluorochem # F492388-25G X-Gal Thermo Fisher # R0404 Oligonucleotides Ultramers IDT N/A Recombinant DNA T7 fluorogenic RNA aptamer library This study N/A J23118 fluorogenic RNA aptamer library This study N/A pBADwt fluorogenic RNA aptamer library This study N/A pBADmut fluorogenic RNA aptamer library This study N/A Htpg1 fluorogenic RNA aptamer library This study N/A M2-VioB-mCherry Ceroni et al 2015 [3] N/A M3-VioB-mCherry Ceroni et al 2015 [3] N/A M4-VioB-mCherry Ceroni et al 2015 [3] N/A pRHA-LacZ Ceroni et al 2018 [19] N/A Software and algorithms FlowJo v10 BD https://www.flowjo.com/ Other Tecan - Infinite M Nano + Monochromator SLS # MIC9266 ClarioSTAR plate reader BMG Labtech Attune CytPix Flow Cytometer Invitrogen Eclipse Ti2-E inverted microscope Nikon onth 2025, Vol. xx, No. xx 17 Experimental model and study participants details Bacterial strains E. coli strains DH10β (K-12 F- λ- araD139 Δ(araA-leu)7697 Δ(lac)X74 galE15 galK16 galU hsdR2 relA rpsL150(StrR) spoT1 deoR φ80dlacZΔM15 endA1 nupG recA1 e14- mcrA Δ(mrr hsdRMS mcrBC)) and BL21(DE3) (F−ompT gal dcm lon hsdSB(rB−mB−) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS)) were used. ..
Software:Article Title: Design of an intracellular aptamer-based fluorescent biosensor to track burden in Escherichia coli.
Article Snippet: .. Key Resources Table REAGENT or RESOURCE SOURCE Trends in Biotechnology, M IDENTIFIER Bacterial and virus strains DH10β NEB # C3019 BL21(DE3) NEB # C2527 Biological samples PURExpress® In Vitro Protein Synthesis Kit NEB # E6800 E. coli S30 Extract System for Circular DNA Promega # L1020 Murine RNase inhibitor NEB # M0314 Chemicals, peptides, and recombinant proteins Chloramphenicol Merck # C1919 Ampicillin Merck # A0166 DFHBI-1T SLS # SML2697-5MG HBC530 Tocris Bioscience # 7277/60U IPTG Merck # I6758 Arabinose Merck # A91906-25G-A HSL Merck # K3007 L-Rhamnose Fluorochem # F492388-25G X-Gal Thermo Fisher # R0404 Oligonucleotides Ultramers IDT N/A Recombinant DNA T7 fluorogenic RNA aptamer library This study N/A J23118 fluorogenic RNA aptamer library This study N/A pBADwt fluorogenic RNA aptamer library This study N/A pBADmut fluorogenic RNA aptamer library This study N/A Htpg1 fluorogenic RNA aptamer library This study N/A M2-VioB-mCherry Ceroni et al 2015 [3] N/A M3-VioB-mCherry Ceroni et al 2015 [3] N/A M4-VioB-mCherry Ceroni et al 2015 [3] N/A pRHA-LacZ Ceroni et al 2018 [19] N/A Software and algorithms FlowJo v10 BD https://www.flowjo.com/ Other Tecan - Infinite M Nano + Monochromator SLS # MIC9266 ClarioSTAR plate reader BMG Labtech Attune CytPix Flow Cytometer Invitrogen Eclipse Ti2-E inverted microscope Nikon onth 2025, Vol. xx, No. xx 17 Experimental model and study participants details Bacterial strains E. coli strains DH10β (K-12 F- λ- araD139 Δ(araA-leu)7697 Δ(lac)X74 galE15 galK16 galU hsdR2 relA rpsL150(StrR) spoT1 deoR φ80dlacZΔM15 endA1 nupG recA1 e14- mcrA Δ(mrr hsdRMS mcrBC)) and BL21(DE3) (F−ompT gal dcm lon hsdSB(rB−mB−) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS)) were used. ..
Flow Cytometry:Article Title: Design of an intracellular aptamer-based fluorescent biosensor to track burden in Escherichia coli.
Article Snippet: .. Key Resources Table REAGENT or RESOURCE SOURCE Trends in Biotechnology, M IDENTIFIER Bacterial and virus strains DH10β NEB # C3019 BL21(DE3) NEB # C2527 Biological samples PURExpress® In Vitro Protein Synthesis Kit NEB # E6800 E. coli S30 Extract System for Circular DNA Promega # L1020 Murine RNase inhibitor NEB # M0314 Chemicals, peptides, and recombinant proteins Chloramphenicol Merck # C1919 Ampicillin Merck # A0166 DFHBI-1T SLS # SML2697-5MG HBC530 Tocris Bioscience # 7277/60U IPTG Merck # I6758 Arabinose Merck # A91906-25G-A HSL Merck # K3007 L-Rhamnose Fluorochem # F492388-25G X-Gal Thermo Fisher # R0404 Oligonucleotides Ultramers IDT N/A Recombinant DNA T7 fluorogenic RNA aptamer library This study N/A J23118 fluorogenic RNA aptamer library This study N/A pBADwt fluorogenic RNA aptamer library This study N/A pBADmut fluorogenic RNA aptamer library This study N/A Htpg1 fluorogenic RNA aptamer library This study N/A M2-VioB-mCherry Ceroni et al 2015 [3] N/A M3-VioB-mCherry Ceroni et al 2015 [3] N/A M4-VioB-mCherry Ceroni et al 2015 [3] N/A pRHA-LacZ Ceroni et al 2018 [19] N/A Software and algorithms FlowJo v10 BD https://www.flowjo.com/ Other Tecan - Infinite M Nano + Monochromator SLS # MIC9266 ClarioSTAR plate reader BMG Labtech Attune CytPix Flow Cytometer Invitrogen Eclipse Ti2-E inverted microscope Nikon onth 2025, Vol. xx, No. xx 17 Experimental model and study participants details Bacterial strains E. coli strains DH10β (K-12 F- λ- araD139 Δ(araA-leu)7697 Δ(lac)X74 galE15 galK16 galU hsdR2 relA rpsL150(StrR) spoT1 deoR φ80dlacZΔM15 endA1 nupG recA1 e14- mcrA Δ(mrr hsdRMS mcrBC)) and BL21(DE3) (F−ompT gal dcm lon hsdSB(rB−mB−) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS)) were used. ..
Inverted Microscopy:Article Title: Design of an intracellular aptamer-based fluorescent biosensor to track burden in Escherichia coli.
Article Snippet: .. Key Resources Table REAGENT or RESOURCE SOURCE Trends in Biotechnology, M IDENTIFIER Bacterial and virus strains DH10β NEB # C3019 BL21(DE3) NEB # C2527 Biological samples PURExpress® In Vitro Protein Synthesis Kit NEB # E6800 E. coli S30 Extract System for Circular DNA Promega # L1020 Murine RNase inhibitor NEB # M0314 Chemicals, peptides, and recombinant proteins Chloramphenicol Merck # C1919 Ampicillin Merck # A0166 DFHBI-1T SLS # SML2697-5MG HBC530 Tocris Bioscience # 7277/60U IPTG Merck # I6758 Arabinose Merck # A91906-25G-A HSL Merck # K3007 L-Rhamnose Fluorochem # F492388-25G X-Gal Thermo Fisher # R0404 Oligonucleotides Ultramers IDT N/A Recombinant DNA T7 fluorogenic RNA aptamer library This study N/A J23118 fluorogenic RNA aptamer library This study N/A pBADwt fluorogenic RNA aptamer library This study N/A pBADmut fluorogenic RNA aptamer library This study N/A Htpg1 fluorogenic RNA aptamer library This study N/A M2-VioB-mCherry Ceroni et al 2015 [3] N/A M3-VioB-mCherry Ceroni et al 2015 [3] N/A M4-VioB-mCherry Ceroni et al 2015 [3] N/A pRHA-LacZ Ceroni et al 2018 [19] N/A Software and algorithms FlowJo v10 BD https://www.flowjo.com/ Other Tecan - Infinite M Nano + Monochromator SLS # MIC9266 ClarioSTAR plate reader BMG Labtech Attune CytPix Flow Cytometer Invitrogen Eclipse Ti2-E inverted microscope Nikon onth 2025, Vol. xx, No. xx 17 Experimental model and study participants details Bacterial strains E. coli strains DH10β (K-12 F- λ- araD139 Δ(araA-leu)7697 Δ(lac)X74 galE15 galK16 galU hsdR2 relA rpsL150(StrR) spoT1 deoR φ80dlacZΔM15 endA1 nupG recA1 e14- mcrA Δ(mrr hsdRMS mcrBC)) and BL21(DE3) (F−ompT gal dcm lon hsdSB(rB−mB−) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS)) were used. ..
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